Showing : 36x three days old zebrafish embryos
Preparation : Mounted live in .25% low melt agarose in a 35 mm ibidi μ dish
Technique : Two brightfield large images consisting of 96 field of views at different Z-sections (7 in total) were recorded (brightfield LED + 488 nm LED).
From the Z sections, an extended depth of field (EDF) projection was processed for each channel. The image shows both channels as a composite.
Microscope : Nikon Eclipse Ti E
Camera : Andor Zyla Plus 4.2 sCMOS
Objective : 10X CFI P-APO ƛ, Immersion = Air, N.A. .45, W.D.[mm] = 4