Teaching

Lectures


Master MSc Cell Biology and Physiology

    • From RNA to Protein, New Sequencing Technologies (Lecture)
    • Nuclear Architecture and Splicing Regulation (Lecture)
    • Nuclear Architecture and Splicing Regulation (Seminar)
 
Link to the Course Catalog.
 
 
 

Courses


2018 - REGISTRATION OPEN-

01.06.-09.06.2018 EMBO Practical Course: iCLIP: Genomic views of protein-RNA, CRG - Centre for Genomic Regulation Barcelona, Spain

2017

26.03.-01.04.2017 EMBO Practical Course: iCLIP: Genomic views of protein-RNA, IMB Mainz, Germany

2016

07.-19.03.2016 Courses@CRG: Post-Transcriptional Gene Regulation (iCLIP & Ribosome Profiling)

2015

16.-21.11.2015 EMBO Practical Course: iCLIP - Genomic views of protein-RNA interactions 


Methods

Our laboratory employs and develops quantitative and genome-wide in vivo approaches to understand the principles of eukaryotic mRNA regulation at the post-transcriptional level through RNA-binding proteins.

 You can learn and apply the following methods in our lab:                       

  • iCLIP-Seq (individual-nucleotide resolution UV cross-linking and Immuno-Precipitation followed by Deep Sequencing)
  • Ribo-Seq (Ribosome footprinting followed by Deep Sequencing)
  • Polysome profiling
  • RNA-Seq
  • Computational analysis of Deep Sequencing data
  • Human and murine cell culture techniques
  • Transfection of mammalian cell lines
  • BAC (bacterial artificial chromosome) recombineering
  • RNA interference (RNAi)
  • Fluorescence microscopy                 
  • Quantitative real-time PCR
  • In-vivo purification of RNA-protein complexes
  • Cloning
  • Western Blotting
  • Northern Blotting