The curriculum of the Master's programme was redesigned in 2015, and all module descriptions are defined within the study regulations from 2015.
The overview below shows the modules accordingly to the semesters they take place. The curriculum offers obligatory courses and optional courses (i.e. elctive modules), enabling PBioC students to select fields matching their profile and interests enhancing their career prospectives.
Familiarity with carrying out well controlled behavioural experiments (animal handling, measuring and analysing behavioural data, statistical analysis). Performing physiological measurements including electrophysiological recording in minimally invasive preparations. Additional aspects are: introduction to software for data handling, signal processing, and graphical display. Deriving scientific questions from the current literature. Knowledge about the usage and limitations of animal models for neurological diseases.
Familiarity with carrying out physiological experiments (animal handling, surgery, measuring and analyzing electrical activity at the single neuron level. Combining physiology with neuroanatomical and histological staining techniques. Basic introduction to behavioural control. Introduction to software for data handling, signal processing, statistical analysis and graphical display. Understanding cognitive influences on sensory information processing as an important aspect of context-dependent behaviour. Deriving scientific questions from the current literature.
Planning, conducting and analysing of behavioural physiological experiments; measuring of ionic currents; behavioural observations and quantifications; neuroanatomical methods. Approaching scientific topics; literature work. Preparing of scientific texts, posters and talks.
The student learns the basic concepts of classical two-dimensional as well as three-dimensional cell culture. She or he is aware of several applications of three-dimensional cell cultures and knows which cell types are used in the Life Sciences. He or she understands the principles of optics in classical microscopy (characteristics of light, resolution, aperture) as well as photometry (energy, power). The student knows the differences between confocal and light sheet-based fluorescence microscopy and is be able to estimate the limits of classical light microscopy in dense tissues. She or he masters the formation, isolation and staining of spheroids, cysts, organoids and three-dimensional tissue slices. The student has experience in the preparation of the specimens for different microscopes as well as the acquisition and processing of the images and the analysis of the data. At the end of the module the student presents the results in a in written report and a talk.
The student learns the principles of insect model organisms, such as Tribolium, in developmental biology. He or she is aware of current scientific questions in developmental biology and knows how to handle transgenic organisms. He or she understands the principles of optics in classical microscopy (characteristics of light, resolution, numerical aperture) as well as photometry (energy, power). The student knows the differences between confocal and light sheet-based fluorescence microscopy and is be able to estimate the limits of classical light microscopy in dense tissues. He or she understands laboratory cultivation of Tribolium as well as preparation methods for confocal and light sheet-based fluorescence microscopes, in the context of long-term live imaging of Tribolium embryos in toto. The student analyses the data and understands the basics of scientific image processing and the embryonic development of Tribolium.
The interns work under guidance on their own individual project based on the actual research topics of the Stelzer group. At the end of the course, they summarize their results and findings in a protocol and prepare a seminar under the guidance of their advisor.
Skills taught: Knowledge to isolate plant cell organelles, independent characterization of organelle proteins, sterile working, culturing and transfection of cells, working with the fluorescence microscope and computational evaluation of experimental data and image files, knowledge in the analysis of transgenic plants, independent handling of scientific questions in the context of relevant scientific literature.
Independent conduct of functional annotation of sequences, of bioinformatics annotation transfer and of prediction of functionally equivalent proteins under consideration of evolutionary relation-ships; Ability for management and bioinformatics analysis of large sequence sets; Mining of public databases; Knowledge of relational database systems; Generation and interpretation of phylogenetic profiles; Introduction into independent scientific research on the background of relevant literature.
The students will learn to plan and to perform complex immunological experiments.
The results of the practical course are presented by every student on the form of a written protocol and a talk at the end of the course. The students also take part on the weekly lab meetings where they learn about the ongoing research of all the members of the group. In a Journal Club every student learns to presents a recent publication on the field of immunology and in context of their own projects.
Students learn the basic techniques to study cellular and molecular aspects of developmental genetics (as detailed above). By the end of the course they have been in direct contact with zebrafish or mice and learn how to handle a zebrafish or mouse colony. The students are in an international environment and learn how to write and communicate their results in English.
This training aims at learning different techniques from the above-mentioned fields, including cell culture of cell lines and primary cells, siRNA-mediated knock-down of genes, preparation of histological sections including staining, confocal microscopy and image analysis, PCR, Western blots, immunoprecipitation, etc.
In the journal club you will present a recent paper. In the end of the course, you will have an opportunity to present your progress during the course. You will work in a very international environment. All of the communication is in English.
Students learn the basic techniques of molecular and developmental biology including zebrafish handling and modern live imaging techniques as detailed above. By the end of the course they have been in direct contact with mice and learn how to handle a mouse colony. The students are in an international environment and learn how to write and communicate their results in English.
Students will be familiarized with scientific literature; will have additional knowledge in RNA biology and special methods of transcript analysis.
They will get practical experience in sterile working with cells and their analysis. At the end of the course they will be able to work with cell cultures for further analysis. They learn how to write, communicate and present their results in English language.
By the end of the course, the students should be able to: (1) Understand basic concepts of bioacoustics such as the sound as a mechanical wave, sound transduction using microphones, analogue-to-digital conversion using sound cards. (2) Measure basic parameters of a sound wave (frequency, duration, intensity). (3) Perform basic surgeries required for acquiring neuronal data. (4) Understand basic neuroscience concepts such as: action potential, local field potential, receptive field, brain topography,
spike clustering, brain oscillations. (5) Testing hypothesis using basic statistical tests (normality tests, parametric and non-parametric t-tests and analyses of variance (ANOVA)).
Students learn the basic techniques for studying cellular, molecular, and systemic neurobiology (as detailed above). They work with cultured cells under sterile conditions, with the epifluorescence microscope and the stereo microscope. The students will be trained in zebrafish embryo handling and basic genetic techniques, and quantify and analyse the obtained data and images. The students are in an international environment and learn how to write and communicate their results in English.
The goal of the module is to provide and extend programming skills, data analysis methods and modelling approaches for dynamical or spatio-temporal processes, or large data sets. Students should be able to apply these methods/approaches in their future research and use them as a foundation for further development. Independent research, usage of original literature, and scientific writing will be strengthened.
During the internship, students learn how genetic manipulation and analysis of a multicellular model organism can help to understand complex cellular relationships that can lead to the development of neurodegenerative disorders.
In this elective module, the student will learn fundamental techniques used to in the research area of neurodegenerative disorders (as described above) by using the mouse model organism. In vivo 2-Photon imaging enables us to record systemic as well as cellular processes in real time. The students are presented with the opportunity to observe in vivo animal handling and the live imaging process. The acquired data will be analyzed by the students, teaching them basic Image- and data analysis skills. The immunohistochemical stainings of brain sections will teach the students the technique as well as the underlying scientific question of the experiment. Moreover, the students will work with cultured cells under sterile conditions, with the epifluorescence – and stereo microscope. The students are in an international environment and learn how to write and communicate their results in English.
Students can plan, carry out and analyse experiments used to investigate psychiatric disorders. Students learn and develop scientific approaches and literature research. The students document their results and communicate them in oral and written form. In a series of seminars (including the opportunity to participate in case presentations), students also acquire basic knowledge of the psychiatric disorders studied and are able to describe them.
The students will learn how to use various CRISPR systems to dissect gene regulatory programs in the cardiovascular system and related cells, which of course can be applied to any other field of research. They learn how to ‘read’ and interpret whole genome data to direct hypothesis on gene regulation and how to design genetic manipulation experiments to verify them.
The practical course teaches students how to implement translational research approaches. In particular, they learn how to use molecular genetic and bioinformatics methods to link findings from the cell model with patient data and vice versa.
Institute of Cell Biology & Neuroscience
Biologicum | Room 2.122
T: +49 69 798-42018
Prof. Dr. Stefan Eimer
Institute of Cell Biology & Neuroscience
Biologicum | Room 2.118
Biozentrum | N101 | Room 1.07
T +49 69 798-46475
F +49 69 798-46470