Model system and methods

lecaudey_bf2x2
As a model organism, we use the zebrafish embryo. This model offers many advantages for research since the embryos can be easily obtained in large numbers and their external development is rapid. Most important, they are fully transparent which enables the observation of the organ development at high resolution and in living organisms.

Main methods that we currently use in the lab:

Live imaging using spinning disc confocal microscopy
primordium_multiple_combined
Generation of mutants using TALEN and Crispr/cas9 system

In situ hybridization and immunostaining
3Dstack ZO1 immunostaining
Quantitative image analysis
stamps zebrafish
3d stamps zebrafish
tracking primordium
rosette detection zebrafish

Contact

Prof. Dr. Virginie Lecaudey
Biologicum | Campus Riedberg
Building part B | Room 2.218
Max-von-Laue-Str. 13
60438 Frankfurt am Main

T +49 69 798-42102
F +49 69 798-42001
E lecaudey@bio.uni-frankfurt.de

Secretary's Office
Birgit Bartussek
Biologicum | Campus Riedberg
Building part B | Room 2.221
Max-von-Laue-Str. 13
60438 Frankfurt am Main

T +49 69 798-42010
F +49 69 798-42011
E bartussek@bio.uni-frankfurt.de