Developmental Biology of Vertebrates

Model system and methods

lecaudey_bf2x2
We use embryos of zebrafish as a model organism. Zebrafish embryos present many advantages for research since they can be easily obtained in large number, there development is fast and important they are fully transparent, making them ideal for looking at the development of organs at high resolution and in living organisms.

Here are the main methods we currently use in the lab:

Live imaging using spinning disc confocal microscopy
primordium_multiple_combined
Generation of mutants using TALEN and Crispr/cas9 system
In situ hybridization and immunostaining
3Dstack ZO1 immunostaining
Quantitative image analysis
stamps zebrafish
3d stamps zebrafish
tracking primordium
rosette detection zebrafish

Contact

VirginieLecaudey_akLecaudey

Prof. Dr. Virginie Lecaudey
Biologicum | Campus Riedberg
Building part B | Room 2.218
Max-von-Laue-Str. 13
60438 Frankfurt am Main

T +49 69 798-42102
F +49 69 798-42001
E lecaudey@bio.uni-frankfurt.de

Secretary's Office
Birgit Bartussek
Biologicum | Campus Riedberg
Building part B | Room 2.221
Max-von-Laue-Str. 13
60438 Frankfurt am Main

T +49 69 798-42010
F +49 69 798-42001
E bartussek@bio.uni-frankfurt.de 

Consultation hours
Mon - Thu 9:00 AM - 1:00 PM